Development and use a Novel Combined In-vivo and In-vitro Assay for Anti-inflammatory and Immunosuppressive Agents

نویسندگان

  • Bo Cao Department of Pharmacognosy, Medical College of the Chinese People’s Armed Police Force, Tianjin, People’s Republic of China.
  • Hong Chen 1- Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Medical College of the Chinese People’s Armed Police Force, Tianjin, People’s Republic of China. 2- Department of Pharmacognosy, Medical College of the Chinese People’s Armed Police Force, Tianjin, People’s Republic of China.
  • Na Wei Department of Pharmacognosy, Medical College of the Chinese People’s Armed Police Force, Tianjin, People’s Republic of China.
  • Tan Li 1- Department of Immunology, Medical College of the Chinese People’s Armed Police Force, Tianjin, People’s Republic of China. 2- Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Medical College of the Chinese People’s Armed Police Force, Tianjin, People’s Republic of China.
  • Xin Mei Department of Pharmacognosy, Medical College of the Chinese People’s Armed Police Force, Tianjin, People’s Republic of China.
چکیده مقاله:

Contact hypersensitivity (CHS) mouse model induced by 2, 4-dinitrofluorobenzene (DNFB) is thought to be a T helper 1 (Th1)-dominant response and used for investigating anti-inflammatory and immunosuppressive agents. However, it is hardly used for screening large-scale drugs because of the large number of animals and complex mechanisms involved in-vivo. In this study, we focused on whether T lymphocytes from CHS mouse model could maintain the state of immune response in-vitro and explored a suitable time for drugs screening. The results showed that CD4+ T cells of CHS mice were higher compared with those in normal group. The expression of T-bet and GATA3 showed a Th1 shift and the levels of interleukin (IL)-2 and IL-4 also showed similar trend. Furthermore, IL-6 produced by T lymphocytes from CHS mice had a high level too. Then, we detected the effects of dexamethasone (DEX), cyclosporine A (CsA) and mycophenolate mofetil (MMF) on T lymphocytes in-vitro, and the data displayed that these immunosuppressive drugs could all inhibit the proliferation of T lymphocytes significantly. These findings suggested that T lymphocytes from CHS mice could mimic a similar immune response in-vitro, and it’s also a suitable method for screening anti-inflammatory and immunosuppressive agents.

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development and use a novel combined in-vivo and in-vitro assay for anti-inflammatory and immunosuppressive agents

contact hypersensitivity (chs) mouse model induced by 2, 4-dinitrofluorobenzene (dnfb) is thought to be a t helper 1 (th1)-dominant response and used for investigating anti-inflammatory and immunosuppressive agents. however, it is hardly used for screening large-scale drugs because of the large number of animals and complex mechanisms involved in-vivo. in this study, we focused on whether t lym...

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Development and Use a Novel combined in-vivo and in-vitro Assay for Anti-inflammatory and Immunosuppressive Agents

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عنوان ژورنال

دوره 12  شماره 3

صفحات  445- 455

تاریخ انتشار 2013-09-01

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